Table 1 FISH probes and ribotags used

From: Primer-free FISH probes from metagenomics/metatranscriptomics data permit the study of uncharacterised taxa in complex microbial communities

FISH probes/ribotags

Intended target taxon

Sequence of probe (5′→3′)

Formamide (%)

References

EUB338

Most bacteria

GCTGCCTCCCGTAGGAGT

0

Amann et al.7

NON338

None

ACTCCTACGGGAGGCAGC

45

Wallner et al.8

Thau646

Thauera

TCTGCCGTACTCTAGCCTT

45

Lajoie et al.43

Ribo_Thau1029_33a

Thauera

GTGTTCTGGCTCCCGAAGGCACCCTCGCCTCTC

N.D

This study

Ribo_Thau1029_17

Thauera

GTGTTCTGGCTCCCGAA

45

This study

Ribo_Unk1029_33a

UPWRP_1

TGCTTCGCGTCTCCGAAGAGCCGACCACCTTTC

N.D

This study

Ribo_Unk1029_17

UPWRP_1

TGCTTCGCGTCTCCGAA

50

This study

Halian2b

Haliangium

CCGACTTCTAGAGCAACTGA

25

McIlroy et al.41

Halian3b

Haliangium

CCAGTCACTCTTTAGGCGGC

25

McIlroy et al.41

Ribo_Halia1029_33a

UPWRP_2

TCTCACTCGCTCCCGAAGGCACCCCGACATCTC

N.D

This study

Ribo_Halia1029_17

UPWRP_2

TCTCACTCGCTCCCGAA

40

This study

Halia183

UPWRP_2

GAAATCCGGAAACCTCACAGAC

40c

This study

  1. N.D not determined
  2. aOriginal 33 bp ribotag of the respective R-Probe
  3. bProbes are components of the HalianMix
  4. cOptimal formamide concentration was not empirically determined