Table 2 Specific thioesterase activity (nmol/min/mg protein) of EB228 and EB228ΔyciA toward acetyl-CoA and butyryl-CoA

From: Discovery of potential genes contributing to the biosynthesis of short-chain fatty acids and lactate in gut microbiota from systematic investigation in E. coli

Substrate

Specific thioesterase activity (nmol/min/mg protein)

Decrease by knockout of yciA

EB228

EB228ΔyciA

Acetyl-CoA

3.3 ± 0.3

3.0 ± 0.5

9%

Butyryl-CoA

5.0 ± 0.3

3.5 ± 0.6

30%

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