Table 1 Quantification of Candida albicans adhesion under flow

From: Candida albicans biofilm development is governed by cooperative attachment and adhesion maintenance proteins

 

2 h Biofilm biomassa (×105)

Attachment rateb (×105)

CDR 0–2 hc

WT

7.18 (1.65)

2.98 (0.01)

0.21 (0.01)

Δ/Δals1/3

2.63 (0.75)

1.18 (0.02)

0.40 (0.02)

Δhyr1

0.82 (0.54)

0.42 (0.01)

0.41 (0.02)

Δhwp2

0.24 (0.29)

0.25 (0.01)

0.58 (0.03)

Δeap1

0.78 (0.23)

0.34 (0.03)

0.13 (0.01)

Δihd1

0.32 (0.27)

0.21 (<0.01)

0.40 (0.02)

WT

7.18 (1.65)

2.98 (0.01)

0.21 (0.01)

Δhog1

4.20 (1.99)NS

1.71 (0.03)

0.19 (0.01)NS

Δsfl1

8.00 (2.16)NS

1.70 (0.06)

0.05 (<0.01)

WT

7.18 (1.65)

2.98 (0.01)

0.21 (0.01)

Δbcr1

7.19 (4.25)NS

3.22 (0.08)

0.47 (0.03)

Δefg1

6.38 (4.42)NS

1.57 (0.02)

0.12 (0.01)

Rapamycin

2.41 (0.87)NS

1.17 (0.03)

0.21 (0.01)NS

WT

7.18 (1.65)

2.98 (0.01)

0.21 (0.01)

Δywp1

8.27 (2.54)NS

1.74 (0.05)

0.07 (<0.01)

  1. Each value represents a mean or best-fit slope of n ≥ 3 experiments, with standard deviation in parentheses
  2. NS indicates no significant difference between each strain compared to wild-type CAI4 cells. All other values were significant (p < 0.05)
  3. aBiofilm Biomass was determined by full frame densitometry analysis at 2 h, with values given in gray values/mm2
  4. bAttachment Rate represents the average biomass of newly attached cells/mm2/h within the first 2 h (the attachment phase). Cell detachment does not impact the attachment rate
  5. cCell Detachment Rate (CDR) was calculated as the average total detachment rate (average biomass of newly detached cells/mm2/h) divided by the biomass of biofilm for the first two hours. Values indicate the average proportion of biomass that detaches from the biofilm per hour
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