Table 3 Detachment rate and adhesion maintenance of Candida albicans biofilms under flow

	Early CDR^a	Late CDR^a	Early AMS^b	Early AMS log₂ (fold change) over WT	Late AMS^c	Late AMS log₂ (fold change) over WT
WT	0.18 (0.01)	0.10 (<0.01)	5.45		9.88
Δ/Δals1/3	0.22 (0.01)^NS	0.17 (<0.01)	4.58^NS	NS	5.97	−0.73
Δhyr1	0.23 (0.01)	0.36 (0.01)	4.33	−0.33	2.81	−1.81
Δhwp2	0.34 (0.02)	0.20 (0.01)	2.98	−0.87	5.08	−0.96
Δeap1	0.27 (0.01)	0.28 (0.01)	3.74	−0.54	3.54	−1.48
Δihd1	0.38 (0.01)	0.13 (<0.01)	2.60	−1.07	7.49	−0.40
WT	0.18 (0.01)	0.10 (<0.01)	5.45		9.88
Δhog1	0.11 (0.01)	0.03 (<0.01)	9.23	0.76	36.76	1.90
Δsfl1	0.04 (<0.01)	0.04 (<0.01)	24.51	2.17	23.20	1.23
WT	0.18 (0.01)	0.10 (<0.01)	5.45		9.88
Δbcr1	0.41 (0.01)	0.21 (<0.01)	2.47	−1.14	4.71	−1.07
Δefg1	0.20 (0.01)^NS	0.20 (<0.01)	5.06^NS	NS	4.98	−0.99
Rapamycin	0.13 (0.01)	0.12 (<0.01)	7.70	0.50	8.22	−0.27
WT	0.18 (0.01)	0.10 (<0.01)	5.45		9.88
Δywp1	0.10 (<0.01)	0.33 (<0.01)	10.40	0.93	3.06	−1.69

Each value represents a best-fit slope of n ≥ 3 experiments, with standard deviation in parentheses. Data was divided into Early (0–5 h) and Late (5–18 h) phases, as many strains displayed an apparent change in rate around 5 h
NS indicates no significant difference between each strain compared to wild-type CAI4 cells. All other values were significant (p < 0.05)
^aCell Detachment Rate (CDR) was calculated as the average total detachment rate (average biomass of newly detached cells/mm²/h) divided by the biomass of biofilm. Values indicate the average proportion of biomass that detaches from the biofilm per hour
^bAdhesion Maintenance Strength (AMS) was calculated as the inverse of the BDR. Value is reflective of the strength with which a cell remains adhered to the cells or substrate of a biofilm over time

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