Table 4 Attachment and detachment of Saccharomyces cerevisiae under flow

From: Candida albicans biofilm development is governed by cooperative attachment and adhesion maintenance proteins

 

Attachment Ratea (×105)

Early CDR (0–10 h)b

Late CDR (10–18 h)b

Early AMS (0–10 h)c

Late AMS (10–18 h)c

ScWT

5.49 (0.12)

0.25 (0.01)

0.47 (0.04)

3.93

3.62

Sc-CaYWP1

8.89 (0.11)

0.28 (<0.01)NS

0.21 (0.01)

2.14

4.72

  1. Each value represents a mean or best-fit slope of n ≥ 3 experiments, with standard deviation in parentheses
  2. NS indicates no significant difference between each strain compared to wild-type cells. All other values were significant (p < 0.05)
  3. aAttachment Rate represents the average biomass of newly attached cells /mm2/h within the first 2 h (the attachment phase). Cell detachment does not impact the attachment rate
  4. bCell Detachment Rate (CDR) was calculated as the average total detachment rate (average biomass of newly detached cells/mm2/h) divided by the biomass of biofilm for the first two hours. Values indicate the average proportion of biomass that detaches from the biofilm per hour
  5. cAdhesion Maintenance Strength (AMS) was calculated as the inverse of the BDR. Value is reflective of the strength with which a cell remains adhered to the cells or substrate of a biofilm over time
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