Table 4 Strains used for this in the present study.

From: Genetic requirements and transcriptomics of Helicobacter pylori biofilm formation on abiotic and biotic surfaces

Strain

KO reference no.

Genotype or description

Reference and/or source(s)

G27

KO379

WT strain

N. Salama (Fred Hutchison Cancer Research Center, USA)

SS1

KO457

WT strain

J. O’Rourke (University of New South Wales, Australia)

J99

KO479

WT strain

N. Salama (Fred Hutchison Cancer Research Center, USA)

26695

KO668

WT strain

ATCC 700392

X472AL

KO612

WT strain

D. Berg (Washington University, USA)

M6

KO613

WT strain

E. Joyce, A. Wright (Tufts University School of Medicine, USA)

CYP34 (CYP3401)

KO637

WT strain

D. Berg

HP43504

KO669

WT/clinical isolate

NTCT 11637

HP 100-1

KO763

WT/clinical isolate

J. Solnick (UC Davis, USA)

HP 12-1

KO760

WT/clinical isolate

J. Solnick

HP 28-1

KO761

WT/clinical isolate

J. Solnick

HP116-1

KO764

WT/clinical isolate

J. Solnick

HP 125-2

KO765

WT/clinical isolate

J. Solnick

HP 208-2

KO767

WT/clinical isolate

J. Solnick

G27 ΔHPG27_166a

KO1635

ΔHPG27_166:aphA3

This study

G27 ΔHPG27_526a

KO1637

ΔHPG27_526::cat

This study

G27 ΔhydEa

KO1638

ΔhydE::cat

This study

G27 ΔHPG27_1233a

KO1639

ΔHPG27_1233::cat

This study

G27 ΔHPG27_1066a

KO1640

ΔHPG27_1066::cat

This study

G27 ΔacsAa,b

KO1641

ΔacsA::cat

This study

G27 ΔacxABa

KO1642

ΔacxAB::cat

This study

G27 ΔHPG27_715a

KO1644

ΔHPG27_715::cat

This study

G27 ΔmotB

KO489

ΔmotB::kan-sac2

This study

G27 ΔfliA

KO1285

ΔfliA::kan

K. Guillemin (University of Oregon, USA)

  1. WT wild-type, aphA3 kanamycin resistant, cat chloramphenicol resistant, ATCC American Type Culture Collection.
  2. aAll transformations were performed using natural transformation using plasmid constructs (pTwist_Amp) in which the gene’s coding sequence was completely or partially removed and replaced by a kanamycin or chloramphenicol-resistance cassette using allelic exchange.
  3. bMutant ΔacsA showed a slight defect in growth.