Table 4 PCR primers used for confirmation of bacterial infection.
From: Polymicrobial periodontal disease triggers a wide radius of effect and unique virome
Bacterium | Primer sequence (5′-3′) |
|---|---|
Porphyromonas gingivalis | F: AGT CGA GTT GCA GAC TCC GAT CC |
R: AAC CCA CAT CGG TAG TTG CTA ACA G | |
Fusobacterium nucleatum | F: AGA GTT TGA TCC TGG CTC AG |
R: GTC ATC GTG CAC ACA GAA TTG CTG | |
Tannerella forsythia | F: T CCC AAA GAC GCG GAT ATC A |
R: ACG GTC GCG ATG TCA TTG T | |
Treponema denticola | F: AGGGATATGGCAGCGTAGCA |
R: TTGCGGGACTTAACCCAACA | |
Universal (total bacteria) | F: TTGGGTTAAGTCCCGC |
R: ATCCCCACCTTCCTCC |
