Fig. 1: Photograph of the monitoring and screening setup. | npj Biofilms and Microbiomes

Fig. 1: Photograph of the monitoring and screening setup.

From: An open-source robotic platform that enables automated monitoring of replicate biofilm cultivations using optical coherence tomography

Fig. 1

Biofilm cultivations are performed in flow cells installed on the experimental layer (1) of the EvoBot (2). Additional equipment such as cultivation media (3), magnetic stirrers (4) and peristaltic pumps (5) are located below the experimental layer. The cultivation media is pumped through the flow cells without recirculation. The OCT scanning probe is installed in the OCT module (6) uniquely provided by the EvoBot platform. A Raspberry Pi (7) controls the EvoBot platform through a graphical user interface and a command line tool. Positioning of the OCT module is either performed manually or automated using scripting. By means of the ThorImage OCT software (8), structural changes in biofilms can be logged directly while running an experiment. The QR code links a video clip showing the EvoBot in action.

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