Fig. 1: Growth characteristics of the wild-type and ΔiolR mutant strains.

Growth curve of the wild-type and ΔiolR mutant strains in LB medium (a) or glucose-free M9 medium supplemented with 50 mM MI (b). c Comparison of colony and microscopic morphologies of strains on LB plates. The colony was observed after culture for 18 h in LB medium (Scale bar = 5 mm). Simultaneously, the microscopic morphology was observed after Gram staining (Scale bar = 50 μm). d Biofilm formation on the flask walls. The bacteria were cultured in LB medium for 18 h at 28 °C. Images were acquired with a camera. e TEM images of the wild-type and ΔiolR mutant. Scale bar = 1 μm. f SEM images of the wild-type and ΔiolR mutant. Scale bar = 4 μm.