Fig. 8: U876_04005 is required for iolR mediated aggregation. | npj Biofilms and Microbiomes

Fig. 8: U876_04005 is required for iolR mediated aggregation.

From: IolR, a negative regulator of the myo-inositol metabolic pathway, inhibits cell autoaggregation and biofilm formation by downregulating RpmA in Aeromonas hydrophila

Fig. 8

a Aggregation of ΔiolRΔU876_13290, ΔiolRΔU876_13510, ΔiolRΔU876_17900, ΔiolRΔU876_19910 and ΔiolRΔU876_04005 was measured macroscopically in stationary tubes after culturing for 7 h. b Ca+-mediated aggregation disappeared in ΔiolRΔU876_04005. Bacteria were grown in LB medium for 18 h, harvested by centrifugation, and then resuspended in PBS supplemented with 10 mM CaCl2. Images of autoaggregation were obtained using a camera after a 2-h incubation. The OD600 of the upper layer of the culture was measured at the same time point. Error bars represent the standard deviations from at least three independent experiments. Statistical significance was determined using a two-tailed unpaired Student’s t test. ***P < 0.001.

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