Fig. 4: Comparison of virulence protein expression in biofilm-isolated (B) and planktonic (P) L. monocytogenes cells. | npj Biofilms and Microbiomes

Fig. 4: Comparison of virulence protein expression in biofilm-isolated (B) and planktonic (P) L. monocytogenes cells.

From: Biofilm-isolated Listeria monocytogenes exhibits reduced systemic dissemination at the early (12–24 h) stage of infection in a mouse model

Fig. 4

a, b Immunoblot (top panel) and densitometry (bottom panels) of InlA, and LAP, in the whole cell (a), cell wall, and intracellular fractions (b) of biofilm-isolated and planktonic cultures of Lm F4244 and F45. Immunoblots are representative of three independent experiments. c Immunoblot of LLO in the secreted protein fraction of biofilm-isolated and planktonic Lm F4244 and F45. Immunoblots are representative of three independent experiments. d Relative mRNA expression of virulence genes (inlA and lap) and virulence regulators (prfA and sigB) in biofilm-isolated and planktonic cells of F4244 and F45 by RT-PCR. The Student’s t-test was used for statistical analysis. ***P < 0.0005; **P < 0.005; *P < 0.05.

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