Fig. 5: Mouse bioassay to compare the pathogenesis of biofilm-isolated sessile and planktonic L. monocytogenes cells.

Lm burden in intestinal (a–d) and extra-intestinal tissues (e–h) after oral inoculation of mice (C57BL/6, male–female, 8–10 weeks old) with 1 × 109 CFU/mouse of sessile (B) and planktonic (P) cells of F4244 or F45 at 24 and 48 hpi. i, j Comparison of the number of bacteria in all intestinal and extra-intestinal tissues at 24 and 48 hpi. Bars represent the median values of each group (B or P). Dashed lines indicate detection limits by a plating method. Mann–Whitney test was used for statistical analysis. **P < 0.005; *P < 0.05.