Fig. 8: Survival and virulence of biofilm-isolated and planktonic L. monocytogenes strain suspended in simulated gastrointestinal fluid. | npj Biofilms and Microbiomes

Fig. 8: Survival and virulence of biofilm-isolated and planktonic L. monocytogenes strain suspended in simulated gastrointestinal fluid.

From: Biofilm-isolated Listeria monocytogenes exhibits reduced systemic dissemination at the early (12–24 h) stage of infection in a mouse model

Fig. 8

a Survival of sessile and planktonic Lm F4244 and F45 after sequential exposure to simulated gastric fluid (SGF, pH 2) for 1 h and simulated intestinal fluid (SIF, pH 7) for 12 h. b–d Comparison of adhesion (b), invasion (c), and translocation (d) rates on Caco-2 cells of SGF (pH 3) and SIF (pH 7)-treated biofilm-isolated and planktonic Lm F4244 and F45. e, f Immunoblot showing LAP and InlA expression in sessile and planktonic cells after exposure to SGF (pH 3) and SIF (pH 7). Immunoblots are representative of three independent experiments. g Relative mRNA expression of virulence genes (inlA and lap) and virulence regulators (prfA and sigB) in biofilm-isolated InlAm from mice intestinal chymus at 12 or 48 hpi and the same cells before infection using RT-PCR. UD, Undetectable. A pairwise Student’s t-test was used for statistical analysis. ***P < 0.0005; **P < 0.005; *P < 0.05.

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