Table 1 Listeria monocytogenes strains (F45, F4244, and F4244 InlA^m) translocation in C57BL/6 mice organs/tissues 12 h after oral infection.

Source	Tissues	Number of mouse tissues positive for L. monocytogenes/# mouse tested (%)^a
		F4244 (WT)		F45 (WT)		F4244 (InlA^m)^b
		Biofilm	Planktonic	Biofilm	Planktonic	Biofilm	Planktonic
Intestinal	Jejunum	0/5 (0)	0/4 (0)	0/5 (0)	0/5 (0)	3/9 (33)	5/9 (56)
	Ileum	0/5 (0)	0/4 (0)	0/5 (0)	0/5 (0)	1/9 (11)	2/9 (22)
	Cecum	1/5 (20)	4/4 (100)	0/5 (0)	4/5 (80)	1/9 (11)	6/9 (67)
	Colon	1/5 (20)	2/4 (50)	0/5 (0)	5/5 (100)	2/9 (22)	5/9 (57)
Extra-Intestinal	MLN	0/5 (0)	3/6 (50)	0/5 (0)	2/5 (40)	0/9 (0)	2/9 (22)
	Liver	0/5 (0)	3/6 (50)	0/5 (0)	2/5 (20)	2/9 (22)	5/9 (56)
	Spleen	0/5 (0)	1/6 (16)	0/5 (0)	3/5 (60)	0/9 (0)	5/9 (56)
	Kidney	0/5 (0)	0/6 (0)	0/5 (0)	0/5 (0)	0/9 (0)	0/9 (0)

^aMice (both male and female) were orally gavaged with 1 × 10⁹ CFU/mouse. Mouse tissue samples were enriched in buffered Listeria enrichment broth for 24 h, plated on modified Oxford agar plate for 48 h, and 1–2 colonies per sample were verified by qPCR (see Supplementary Table 2).
^bThese animals received streptomycin (5 mg/ml) in water for 32 h, followed by 16 h antibiotic-free water before oral gavage with Lm.

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