Fig. 6: G-quadruplex DNA present in static and flow-cell P. aeruginosa biofilms. | npj Biofilms and Microbiomes

Fig. 6: G-quadruplex DNA present in static and flow-cell P. aeruginosa biofilms.

From: The biofilm matrix scaffold of Pseudomonas aeruginosa contains G-quadruplex extracellular DNA structures

Fig. 6

Three-dimensional (3-D) confocal micrographs of 3-day P. aeruginosa wild-type flow-cell biofilm showing the colocalisation of eDNA and G-quadruplex nucleic acid structures in the matrix of the biofilm, as illustrated by binding to DNA-specific dye propidium iodide (PI; red), (A) the binding of anti-DNA G-quadruplex structure antibody to the eDNA fibres, visualised with GFP-labelled goat anti-mouse IgG (green) (B) and overlapping binding of both PI and anti-DNA G-quadruplex antibody (C). The zoomed-in insets from the three images showing a region of colocalisation of G-quadruplex antibody with PI.

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