Fig. 1: Assessment of myeloid clustering in chronic infection. | npj Biofilms and Microbiomes

Fig. 1: Assessment of myeloid clustering in chronic infection.

From: Treatment with a neutrophil elastase inhibitor and ofloxacin reduces P. aeruginosa burden in a mouse model of chronic suppurative otitis media

Fig. 1

The mouse CSOM middle ear effusion was assessed at 14 d.p.i. by FCM. Top panels: analysis of control mice. Bottom panels: analysis of infected mice. Tree-structured visualization of clusters by minimal spanning tree (MST), with cluster sizes correlating with the number of cells within the cluster (a control; c infected). Each point in the tree indicates single populations according to fluorescence intensity, and comparison of subpopulations in effusion from infected c.f. control animals. We manually annotated subpopulations in (c) for visual clarity. Minimal spanning tree diagram shows dimensionality reduction of cellular infiltrate in CSOM mice at 14 d.p.i. Representative MS trees of one uninfected (a) and one CSOM (c) sample. (n = 3–12 mice per group). t-SNE maps at 14 d.p.i. are shown with each color representing different subpopulations (1–12) based on scatter and fluorescent intensity (b control; d infected). Region size of the colored subpopulations is representative of increased cell density for the populations.

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