Fig. 3: Functional analysis of annotations isolated by the keyword “virulence”.

a Bar chart showing the top 10 taxa by with virulence annotations. Annotations were extracted using the “subsetTax” tool included with the SQMtools R package. Patients 16 and 11 had no annotations related to “virulence” within these samples. b Heat map of the top ten most enriched virulence proteins across all PEDIS infection severities. Read counts were normalized prior to applying a multilevel analysis (ANOVA) fitting all contrasts between groups to identify the top ten enriched genes. Patients are grouped by infection severity (PEDIS) with features clustered using Ward clustering.