Fig. 3: Protein S-nitrosylation profiles in E. coli planktonic (PK) or biofilm (BF) conditions. | npj Biofilms and Microbiomes

Fig. 3: Protein S-nitrosylation profiles in E. coli planktonic (PK) or biofilm (BF) conditions.

From: Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress

Fig. 3

Cultures were grown under aerobiosis (+O2) or anaerobiosis (−O2) and under S-nitrosylating conditions with excess 10 mM nitrate (S-NO+), or non-S-nitrosylating condition supplemented with 10 mM fumarate (S-NO−). Proteins were extracted, processed with the biotin-switch method (see Fig. 1) and analyzed by western blot. A Total protein extracts visualized with Stain-free fluorescent detection; B S-NO proteins visualized by western blot followed by immunodetection using avidin-HRP antibodies.

Back to article page