Fig. 4: Evaluation of the ability of insect-specific viruses to cross-infect the cryptic whitefly species Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED). | npj Biofilms and Microbiomes

Fig. 4: Evaluation of the ability of insect-specific viruses to cross-infect the cryptic whitefly species Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED).

From: Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci

Fig. 4

a Diagram illustrating the experimental workflow. In the upper panel whitefly individuals of NBU-Q (representing cryptic species MED) were microinjected with homogenate of NBU-B (representing cryptic species MEAM1). The reciprocal injection of NBU-B with homogenate of NBU-Q whiteflies is shown in the lower panel. b Detection of insect-specific viruses (ISVs) 0, 3, 6, and 12 days post injection (DPI), and in the next generation (F1). The presence of ISVs in each whitefly sample was determined by RT-PCR. Untreated NBU-B and NBU-Q whiteflies were used as controls. A pool of 20–30 whiteflies were collected for MEAM1 and MED whiteflies at each time point, and three independent biological replicates were performed. Abbreviation of virus names: BtArV2, Bemisia tabaci arlivirus 2, BtPiLV1 Bemisia tabaci picorna-like virus 1, BtQuV1 Bemisia tabaci quaranjavirus 1, BtViLV1 Bemisia tabaci virga-like virus 1, BtViLV2 Bemisia tabaci virga-like virus 2.

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