Fig. 6: Spatial transition to the channel edge.
Fluorescence images of Bt (a) and Pf (b) populations in the 4S biofilm, taken in the initial colonization phase (left image) and after the spatial transition (right image); scale bar represents 100 µm. The small bottom images are zoomed-in details showing local heterogeneities of the globally uniform distribution in the initial colonization phase; scale bars represent 5 µm. Graph of the ratio as a function of the fluorescence intensity time at the channel edge (Fledge) to that at the channel center averaged on ROIs (20 µm wide) located at 20 and 360 µm from the channel wall, respectively; Bt in green and Pf in red (c, upper panel). Kymographs of Bt (green blue) and Pf (Red) spatial distribution are presented (c, lower panel). d Detection of Kv cells and clusters in the 4S biofilm using the NIS dark-spot detection tool (details provided in the Supplementary Note 3). Images correspond to time t = 14 h. In the bottom panel, the detection results are overlaid on the original image shown in the upper panel; scale bar is 50 μm. e Kv spots were then counted in 30-μm wide ROIs from the edge to the center of the channel for time t = 10 min (⎕); 20 min (●); 7h30 (×); 6h30 (▢); and 32 h (+). Error bars report the error of 10% made on spot counts as evaluated by comparing automatic and visual detection.
