Fig. 7: Species exhibit distinct local dynamics in the 4S community.

a The consecutive frames correlation coefficient calculated from time-lapse recordings of Bt-FAST (green curve) and Pf-mCherry (red curve) in the 4S biofilm community. Colocalization maps of Bt (b) and Pf (c) cells reveal spatial self-overlap between time t and t′ = t + 10 min, taken at t = 1 h (upper panels) and t = 3 h (lower panels). Light gray pixels correspond to non-moving cells (pixels unchanged between t and t′), while blue pixels correspond to newly appeared cells (pixels that were dark at t and subsequently light at t′), and magenta pixels correspond to removed cells (pixels that were light at t and subsequently dark at t′); scale bar represents 10 µm. d Confocal images of Pf-mCherry in the 4S community taken at t = 6h40 (left upper); 7 h (right upper); 7h30 (left lower); and 8 h (right lower), showing full detachment of the cell groups; see also colocalization maps in Supplementary Fig. 3; scale bar represents 5 µm. e Transmitted light images of the 4S biofilm taken at time t = 1 h (left); 12 h (center); and 37 h (right), showing fixed localizations of Kv (indicated by the yellow arrows) all throughout the biofilm growth; scale bar represents 5 µm. f Successive frames (left upper; left lower, right lower) of the 4S biofilm in transmitted light and red fluorescence taken from t = 1 h (left upper) at 10-min intervals. The upper right frame is a zoom in of left upper quadrant. Yellow squares indicate the Rh cells, illustrating their high dynamics with different positions on each frame; scale bar represents 10 µm.