Fig. 3: Anti-Psl antibody binding. | npj Biofilms and Microbiomes

Fig. 3: Anti-Psl antibody binding.

From: The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis

Fig. 3

SickKids P. aeruginosa isolates (N = 7 Persistent; N = 7 eradicated), EPIC P. aeruginosa isolates (N = 7 Persistent; N = 7 eradicated), laboratory P. aeruginosa strain PAO1 and Δpsl (Psl deficient PAO1) were grown as biofilms for 48 h then incubated for 90 min with fluorescently labeled monoclonal anti-Psl antibodies, A Psl0096, B WapR001, and C WapR016. The mean antibody fluorescence volume (µm3/100,000 µm3 biofilm) for each isolate (done in triplicate) was calculated and the mean of all isolates was plotted with standard error of the mean (SEM). Statistics were performed using non-parametric Mann–Whitney test ***p < 0.001, **p < 0.01, *p < 0.05, ns not significant.

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