Fig. 4: SickKids P. aeruginosa isolates (N = 7 persistent; N = 7 eradicated) were grown as biofilms for 24 h after which antibodies (LB control, IgG or anti-Psl 0096) and tobramycin 1000 µg/ml (or LB alone) were added for the following 24 h before imaging was performed. | npj Biofilms and Microbiomes

Fig. 4: SickKids P. aeruginosa isolates (N = 7 persistent; N = 7 eradicated) were grown as biofilms for 24 h after which antibodies (LB control, IgG or anti-Psl 0096) and tobramycin 1000 µg/ml (or LB alone) were added for the following 24 h before imaging was performed.

From: The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis

Fig. 4

A The mean biofilm volume (measured in µm3) for each isolate (done in triplicate) was calculated and the mean of all isolates was plotted with standard error of the mean (SEM). Comparisons with and without tobramycin treatment were performed for each condition using non-parametric Mann–Whitney test ***p < 0.001, **p < 0.01, *p < 0.05, ns not significant. B In the presence of tobramycin, aggregation was measured using biofilm surface to volume ratio. The mean biofilm surface to volume ratio (µm2/µm3) was calculated for each isolate (done in triplicate) and the mean of all isolates was plotted with standard error of the mean (SEM). Comparisons to IgG control were performed for each condition using non-parametric Mann–Whitney test ***p < 0.001, **p < 0.01, *p < 0.05, ns: not significant. C Representative images are shown for a persistent (Pa375) and eradicated (Pa558) isolate.

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