Fig. 3: Effects of benthic organism and nutrient enrichment on biofilm communities within each time point.

At each time point, both nutrient treatment and benthic organism significantly influence biofilm community structure (p < 0.001). Organism (a–c) is a better predictor of biofilm community than nutrient level (d–f) across each time point. A significant interaction between organism and nutrient level is also observed at each time point: 2 week (R2 = 0.140, p = 0.003), 4 week (R2 = 0.144, p = 0.031) and 6 week (R2 = 0.156 & p = 0.006), indicating that nutrients affected community structure differentially according to the benthic organism present.