Fig. 3: Binding of DC-SIGN, MR-CTLD4-7 and Dectin-2 to P. aeruginosa biofilm carbohydrates. | npj Biofilms and Microbiomes

Fig. 3: Binding of DC-SIGN, MR-CTLD4-7 and Dectin-2 to P. aeruginosa biofilm carbohydrates.

From: Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function

Fig. 3

a–c. Lectin competition assays demonstrate dose-dependent inhibition of fucose-PAA (2 µg/ml) binding to DC-SIGN (a) and MR-CTLD4-7 (b), and of zymosan (1 × 106 particles) to Dectin-2 (c), by HMW-1. Fc-chimeric proteins and anti-human Fc-secondary antibody conjugated to alkaline phosphatase were used. Graphs show mean ± SEM of 4 independent repeats done in duplicate. Analysis was performed using One-way ANOVA corrected for multiple comparisons using the Dunnett’s multiple comparisons test. ****, <0.0001; ***, 0.0007; **, 0.007, *, 0.0493. d HMW-2 binds rhDC-SIGN, rhMR, and rhDectin-2. Tetrameric hDC-SIGN, biotinylated and immobilised on a streptavidin sensor and rhMR and rhDectin-2 immobilised on a Ni-sensor were incubated with different HMW-2 concentrations. The table shows equilibrium dissociation constants for the receptor ligand interaction in μM (KD); receptor density on the biosensor surface (BMAX) and nonspecific binding (KNON-SPECIFIC). 95% confidence intervals in µM are shown within brackets.

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