Fig. 5: HMW-2 inhibits Dectin-2-mediated cellular activation.

a HMW-2 does not induce Dectin-2 activation. Dectin-2 reporter cells (HEK-blue muDectin-2) and control cells (Null 1 V) (5 × 10⁴ cells per well) were exposed to different stimuli [LPS (1 and 10 µg/ml), Zymosan (1 and 100 µg/ml), laminarin (100 and 1 µg/ml) and LPS-free HMW-2 (100, 10, 1, and 0.1 µg/ml)] for 18 h. Plates were read at OD_{645 nm}. Figure shows representative image from two independent repeats. b. HMW-2 acts as an antagonist for Dectin-2. Dectin-2 reporter cells (5 × 10⁴ cells per well) were preincubated with 0.3 µg/ml of LPS-free HMW-2 for 1 h and then exposed to different zymosan doses for 18 h. Plates were read at OD_{645 nm}. The figure shows representative results from 3 independent repeats.