Fig. 2: Distribution of bi-modal gene expression across 414 clinical P. aeruginosa isolates. | npj Biofilms and Microbiomes

Fig. 2: Distribution of bi-modal gene expression across 414 clinical P. aeruginosa isolates.

From: Evolution of biofilm-adapted gene expression profiles in lasR-deficient clinical Pseudomonas aeruginosa isolates

Fig. 2

Multimodal (R-package ‘multimode’, adjusted p-value ≤ 0.1) gene expression distributions were recorded, and the distribution of the 136 bi-modal genes (y-axis), which showed high expression (red) or a low expression (blue) across the 414 clinical isolates (x-axis), was recorded. The resulting binary matrix was used as a basis for a heat map (binary clustering of rows and columns). Clinical isolates harboring non-synonymous mutations in lasR are marked in green, insertions/deletions/premature stop codons (InDel/STOP) are marked in yellow. Clustering uncovered 5 groups of genes with a similar bi-modal expression pattern in the clinical isolates. They were colored on the y-axis for a better overview (Gene Cluster 1-5). Only bi-modally expressed genes that are assigned in both reference strains, PA14 and PAO1 were included.

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