Fig. 1: Structural features of monomeric SslE.
From: Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation
a Schematic of SslE from E. coli W with mature sequence numbers and structural features annotated. SS: periplasmic signal sequence; L: flexible linker; NT: unique N-terminal region; M60: peptidase/mucinase domain. b SAXS bead model of rSslE at pH 7.4. c Fit of the SAXS bead model (teal line) and negative-stain TEM map (black line) of rSslE to the rSslE SAXS data (black open circles) with χ2 of 1.2 and 113.7, respectively. d Representative negative-stain TEM micrograph of rSslE (pH 7.4) at ×50,000 nominal magnification with representative 2D classifications. Scale bar represents 20 nm. e Overlay of the 22 Å resolution TEM map (grey) and SAXS bead model (teal) with the three defined regions in SslE highlighted. f Docking of an SslE M60 domain homology model (orange) into the TEM map. g TEM map of rSslE coloured based on domain organisation. Possible alternative assignments of the NT1 and NT2 domains in regions 1 and 2, and the NT3 and M60 domains in region 3 are shown in parentheses. The NT3-M60 interdomain channel is highlighted with an arrow. h TROSY 1H15N-HSQC spectrum of rSslE (black) overlaid with 1H15N-HSQC spectra of the SslE NT1 (light green) and NT2 (olive green) domains.
