Fig. 5: Analysis of SslE-dependent biofilm formation by E. coli W strain.
From: Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation
a Macrocolony biofilm phenotype of wild-type E. coli W and derivatives on LB Congo red agar medium after 24 and 96 h of growth. The derivatives sslE and sslEΔM60 represent trans-complementation of intact sslE (ΔsslE::sslE) or sslE minus its M60 domain (ΔsslE::sslEΔM60), respectively, into the E. coli W ΔsslE mutant. Scale bar = 5 mm. b Zoomed-in image of the wild-type E. coli W and ΔsslE mutant. Scale bar = 2 mm. c CLSM images of wild-type E. coli W biofilms and E. coli W ΔsslE mutant and trans-complementation with sslE and sslEΔM60 stained with FM 1-43 (green). Scale bar represents 20 μm. d The pH across wild-type E. coli W biofilms grown for 96 h was monitored ratiometrically using C-SNARF-4 (green). pH values were calculated over ~30 μm2 boxes and pH values for representative regions within the biofilm fringes and centres are annotated. Dotted line outlines water channel within the biofilm, where pH values are not shown. Scale bar represents 20 μm. All data are representative of at least three independent experiments.
