Fig. 6: EPS production in E. coli macrocolony biofilms.

Analysis and quantification of EPS from macrocolony biofilms of wild-type E. coli W, BL21 (DE3), H10407 E2348/69 and BW25113 strains and their derivatives after 96 h growth. a Macrocolony phenotypes of strains grown at 37 °C on LB Congo red agar medium, b 25 °C on LB Congo red agar medium and 25 °C on YESCA Congo red agar medium. Scale bar represents 5 mm. c Cellulose production after digestion with cellulase from strains grown on LB agar (grey) or YESCA agar (yellow) at 37 °C (no asterisks) or 25 °C (*). d Immunoblot analysis of whole cells grown on LB agar (grey line) or YESCA agar (yellow line) at 37 °C (no asterisks) or 25 °C (*), detected with either anti-rSslE antibody or anti-CsgA antibody. e Dot-blot analysis of PNAG content from whole cells grown on LB agar at 37 °C (no asterisks) or 25 °C (*), detected with wheat germ agglutinin (WGA). f Quantification of colanic acid from strains grown on LB agar (grey line) or M9-glucose agar (blue line) at 37 °C (no asterisks), 25 °C (*) or 18 °C (**). Data are representative of at least three repeat experiments. All blots were derived from the same experiment and processed in parallel and error bars are the standard error of the mean.