Fig. 2: Contribution of genes encoding previously uncharacterized proteins to biofilm drug tolerance.

Susceptibility phenotype of 2 day-old biofilms by wild type and transposon mutants to a tobramycin (150 µg/ml) and b 0.6% hydrogen peroxide. Biofilms were exposed to tobramycin or hydrogen peroxide for 1 h under flowing conditions. Viability was determined via CFU counts. Susceptibility is expressed as log10 reduction in viability. Experiments were performed in triplicates, with two technical replicates each. Error bars indicate standard deviation. Dashed lines indicate log reduction noted for wild-type biofilms. **, significantly different from PAO1; p < 0.1, as determined by ANOVA followed by a Dunnett’s post-hoc test.