Table 3 COMSTAT 2.0 quantitative analysis of CSLM images of biofilms formed by indicated strains.

From: A previously uncharacterized gene, PA2146, contributes to biofilm formation and drug tolerance across the ɣ-Proteobacteria

 

Biomass (µm3/µm2)

Thickness (µm)

P. aeruginosa

PAO1

6.52 (±3.26)

5.82 (±5.31)

PAO1/pMJT-1

7.28 (±3.1)

8.25 (±13.12)

PAO1/pMJT- PA2146

13.84 (±7.16)*

15.61 (±26.32)*

ΔPA2146

1.09 (±0.97)

1.4 (±1.61)

ΔPA2146/pMJT-1

0.46 (±0.18)*

0.51 (±1.3)*

ΔPA2146/pMJT-PA2146

5.43 (±3.29)

4.76 (±7.63)

ΔPA2146/pMJT-ymdFEc

9.02 (±6.4)

11.41 (±9.36)

ΔPA2146/pMJT-ymdF1Kp

10.35 (±2.72)

15.32 (±10.50)

ΔPA2146/pMJT-ymdF2Kp

11.01 (±3.57)

13.94 (±5.37)

ΔPA2146/pMJT-ymdF3Kp

8.30 (±3.77)

9.82 (±4.63)

ΔPA2146/pMJT-sagS

1.76 (±2.3)*

1.95 (±8.29)*

ΔsagS

0.82 (±0.63)*

0.83 (±2.88)*

ΔsagS/pMJT-1

0.62 (±0.5)*

0.52 (±3.61)*

ΔsagS/pMJT-PA2146

9.24 (±6.4)

8.96 (±23.64)

ΔsagS/pMJT-ymdF

7.76 (±5.44)

11.99 (±23.01)

ΔsagS/pMJT-ymdF1Kp

8.18 (±3.34)

9.52 (±4.22)

ΔsagS/pMJT-ymdF2Kp

8.28 (±2.39)

9.27 (±2.85)

ΔsasgS/pMJT-ymdF3Kp

9.67 (±3.19)*

11.27 (±4.48)*

E. coli

BW25113

7.22 (±1.51)

8.70 (±1.76)

ΔymdFEc

1.19 (±0.75)*

0.94 (±0.64)*

K. pneumoniae

MKP103

5.36 (±1.85)

6.57 (±3.29)

ymdF1Kp∷T30

1.76 (±0.77)*

3.97 (±2.33)*

ymdF2Kp∷T30

0.60 (±0.49)*

1.07 (±1.09)*

ymdF3Kp∷T30

0.31 (±0.32)*

0.81 (±1.65)*

  1. Experiments were performed in triplicate, with each biological replicate being represented by a minimum of 8 confocal images taken at random. Standard deviation is indicated in parenthesis. *Significantly different from respective parental background (PAO1, PAO1/pMJT-1, BW25113, or MKP103); p < 0.05, as determined using 1-way ANOVA, followed by a Dunnett’s post-hoc test.
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