Fig. 5: Biofilm evolution.

a Scheme of the deposition of L. parabuchneri biofilms at the ATR waveguide surface after the conditioning film was formed. b Optical image of L. parabuchneri bacterial biofilm attached to the surface of the ATR waveguide during 7 days of IR measurements at flow-through conditions. c 3D plot of IR-ATR spectra vs. time revealing the molecular changes occurring during 72 h of biofilm development. d Concentration profiles of oxygen prior to and after inoculation of the L. parabuchneri suspension.