Fig. 6: IR-ATR spectra of L. parabuchneri biofilms monitored for 24, 48, and 72 h during real-time incubation within the flow system.

The observed spectral changes correspond to proteins (amide I: 1700–1618 cm−1, amide II: 1585–1486 cm−1), LA: 1465–1293 cm−1, amide III + NA + PL: 1350–1180 cm−1 and PS + NA + PL: 1189–960 cm−1, respectively (LA = lactic acid or lactate, NA = nucleic acids, PL = phospholipids, PS = polysaccharides).