Fig. 7: Deconvolution of IR spectra for unraveling molecular details during biofilm formation.

a IR-ATR spectrum of MRS medium supplemented with 5 mM L ( + ) L-histidine (red) and experimentally obtained spectra of the individual constituents in the spectral region from 1800 to 900 cm⁻¹ (see color code, top, middle). b Resulting cumulative impulse fit (black) and fitted IR signatures of the individual constituents (see color code, top, right). The integration values (peak center and width) of the individual components (see Table 2) served as restrictions for the cumulative impulse fit (n ≥ 10; Gaussian fit). The experimentally obtained sum spectrum is given in red in (a) and (b).