Fig. 8: NucB exerts antibiofilm activity in the presence or absence of sucrose. | npj Biofilms and Microbiomes

Fig. 8: NucB exerts antibiofilm activity in the presence or absence of sucrose.

From: Interspecies competition in oral biofilms mediated by Streptococcus gordonii extracellular deoxyribonuclease SsnA

Fig. 8

a Oral microcosm biofilms grown for 20 h in natural saliva and supplemented with NucB in the lower of the microfluidic channel. Yoyo-1 (2.4 nM) dye was used for eDNA visualisation. b eDNA quantified in the two halves of the growth channel over 20 h of growth. eDNA was measured through GrayValue determination in FIJI software for images taken every 10 min. c Sucrose (0.3%) supplemented saliva was used to grow microcosm biofilms and NucB was included in the lower half of the growth channel. d eDNA quantified for the NucB-containing and NucB-free halves of the growth channel over 20 h of growth in sucrose (0.3%) containing saliva. Mean and SE from 3 independent repeats are shown. Scale bars are 50 µm.

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