Table 1 Primers used for real-time qPCR.
From: Chicken cecal microbiota reduces abdominal fat deposition by regulating fat metabolism
Gene | Forward Sequence (5′-3′) | Reverse Sequence (5′-3′) | Gene Bank No. |
|---|---|---|---|
β-actin | TTGTTGACAATGGCTCCGGT | TCTGGGCTTCATCACCAACG | NM_205518.2 |
ACC | TCCAGCAGAACCGCATTGACAC | GTATGAGCAGGCAGGACTTGGC | NM_205505.2 |
FAS | GCTCTGCGTCTGCTTCAGTCTAC | GGTACAGGACTCTGCCATCAATGC | NM_205155.4 |
LPL | TGGACATTGGTGACCTGCTTATGC | TCGCCTGACTTCACTCTGACTCTC | NM_205282.2 |
ACSL1 | GACTAATGGTCACAGGAGCAGCAC | CCAGGCATTGACAGTGAGCATCC | NM_001012578.2 |
FADS1 | CCGTGCCACTGTGGAGAAGATG | GCCTAGAAGCAACGCAGAGAAGAG | XM_040673219.1 |
CYP2C45 | AACAAGCACCACCACACGATACG | GGTCAGCCACGCAAGGTCTTC | NM_001001752.3 |
APOAI | GTGACCCTCGCTGTGCTCTT | CACTCAGCGTGTCCAGGTTGT | NM_205525.5 |
PPARα | TGCTGTGGAGATCGTCCTGGTC | CTGTGACAAGTTGCCGGAGGTC | XM_040699549.1 |
CPT-1 | ACAGCGAATGAAAGCAGGGT | GCCATGGCTAAGGTTTTCGT | NM_001012898.1 |
LEPR | CACTCGCTGGGAACACTTGA | TTCAGCAGCCCATCGTTTCT | NM_204323.2 |
JAK2 | GAGCGTGAGAATGCCACTGAC | TGGAGGACAGCACTTGATGAAC | NM_001030538.3 |
STAT3 | GCCGAATCACAACTACAGACTC | CTGACTTTGGTGGTGAACTGC | NM_001030931.3 |
HSL | GAGGCACAGCGTCTTCTTTAGG | GGCACGAACTGGAACCCGAG | XM_040695201.1 |
