Fig. 1: B. uniformis JCM5828 ameliorates DSS-induced colitis by protecting against intestinal barrier damage and tight junction disruption. | npj Biofilms and Microbiomes

Fig. 1: B. uniformis JCM5828 ameliorates DSS-induced colitis by protecting against intestinal barrier damage and tight junction disruption.

From: Bacteroides uniformis-induced perturbations in colonic microbiota and bile acid levels inhibit TH17 differentiation and ameliorate colitis developments

Fig. 1

a Schematic of the experimental design. Mice (female, n = 16 per group) were given 3.0% DSS for 24 d and treated with PBS or B. uniformis JCM5828 from d 15 to d 24. The number of mice surviving in the Con group on the day of sampling (n = 8) defined the final biological replicate of each group. b Changes in body weights during the experiments (n = 8). c DAI scores during the experiments (n = 8). d Survival curve during the experiments (n = 16). e A representative image of colon tissue from each group was provided, and the colon length was recorded (n = 8). f The spleen weight of each group (n = 8). g The histological score of the DSS-induced colitis was evaluated. h Representative microscopic images of mouse colon tissue stained with H&E and PAS (Scale bars = 200 μm) and corresponding local high magnification images (Scale bars = 50 μm). i The goblet cell count of the DSS-induced colitis was evaluated (n = 5). j qPCR analysis showing the mRNA expression of Occludin, ZO-1, Claudin-1 in colon tissues (n = 8). **p < 0.01, ***p < 0.001. Data were analyzed using one-way ANOVA with Tukey’s test and expressed as the means ± SEM.

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