Fig. 4: B. uniformis JCM5828-mediated bile acids inhibit TH17 differentiation and alleviate DSS-induced colitis. | npj Biofilms and Microbiomes

Fig. 4: B. uniformis JCM5828-mediated bile acids inhibit TH17 differentiation and alleviate DSS-induced colitis.

From: Bacteroides uniformis-induced perturbations in colonic microbiota and bile acid levels inhibit TH17 differentiation and ameliorate colitis developments

Fig. 4

a Gating strategy for the flow cytometric sorting of intestinal CD4+ T cells. Flow cytometric analyses (b) and quantification (c) of RORγt production from C57BL/6J mice naive T cells cultured for 3 d under the TH17 cell polarization condition. PBS, PBS, or Bu supernatant (MOI = 1:10) were added after LPS addition (n = 3 respectively, biologically independent samples). d Gating strategy for the flow cytometric sorting of intestinal CD4+ T cells. Flow cytometric analyses (e) and quantification (f) of RORγt production from C57BL/6J mice naive T cells cultured for 3 d under the TH17 cell polarization condition. DMSO, Bu supernatant (MOI = 1:10), or bile acids (20 μM) were added after cytokine addition (n = 3 respectively, biologically independent samples). Data were analyzed using one-way ANOVA with Tukey’s test and expressed as the means ± SEM. ***p < 0.001.

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