Fig. 6: The mixed bile acids inhibits TH17 differentiation.

a Gating strategy for the flow cytometric sorting of intestinal CD4+ T cells. Flow cytometric analyses (b) and quantification (c) of Th17 (CD3+CD4+RORγt+) frequencies in the colonic lamina propria of C57BL/6J mice in each group (n = 3 respectively, biologically independent samples). Data were analyzed using one-way ANOVA with Tukey’s test and expressed as the means ± SEM. ***p < 0.001.