Fig. 3: Changes in cytokine expression in peripheral blood mononuclear cells (PBMCs) following treatment with F. periodonticum alone (red), or in combination with B. fragilis (blue) or P. asaccharolytica (yellow) for cytokines IL-18, IL-10, and IL-1β.
From: Bacterial lipopolysaccharide modulates immune response in the colorectal tumor microenvironment
For these experiments we used the lowest concentration of F. periodonticum LPS (6 ng/mL) and the highest concentration of LPS from B. fragilis or P. asaccharolytica (600 ng/mL), as these respective concentrations had the largest effects on cytokine production in earlier experiments. Values are shown as percentages of PBMC baseline secretion, which is set at 100%. Dashed lines indicate a single experimental run. Colored, solid horizontal lines represent the means of repeat experiments. Y-axes of IL-10 and IL-1β are in log10 scale, while Y-axis of IL-18 is in the linear scale. Fpe = F. periodonticum (6 ng/mL), Fpe + Bfr = F. periodonticum (6 ng/mL) + B. fragilis (600 ng/mL), Fpe + Pas = F. periodonticum (6 ng/mL) + P. asaccharolytica (600 ng/mL); * = Paired Student’s t tests p-value < 0.05 (F. periodonticum vs PBMC, F. periodonticum + B. fragilis vs F. periodonticum alone, or F. periodonticum + P. asaccharolytica vs F. periodonticum alone).
