Fig. 7: The effect of core metabolites generated during fermentation of the preome architecture, and biofilm formation. | npj Biofilms and Microbiomes

Fig. 7: The effect of core metabolites generated during fermentation of the preome architecture, and biofilm formation.

From: Metabolic inputs in the probiotic bacterium Lacticaseibacillus rhamnosus contribute to cell-wall remodeling and increased fitness

Fig. 7

A Heat map showing the mass-spec intensities of all the proteins identified in proteomics analysis of LGG grown in liquid TSB medium, TSB medium supplemented with glucose (1% W/V), TSB medium supplemented with 20 µM acetic acid (AA) or TSB medium supplemented with 10 µM acetyl-CoA (A-CoA). Volcano plot representing the log2 fold-change (AA/TSB) against -log10 statistical p-value for proteins differentially expressed between (B) acetic acid vs. control (TSB) and C acetyl-coA vs. control (TSB). Colored dots represent the GO categories of differentially expressed proteins. The horizontal dashed line indicates p = 0.05 and vertical dashed line indicates a log2FC of |0.5|. D LGG cells were diluted 1:100 into a fresh TSB medium or TSB supplemented with different concentrations of acetic acid, E acetyl-CoA, F pyruvate, and G lactic acid. 200 μL of cultures were split into a 96-well polystyrene plate and further incubated at 37 °C. Crystal violet assay was used to assess the biofilm formation of LGG after 72 h. Graph represents the mean ± SD from three biological repeats. Statistical analysis was performed using Brown-Forsythe and Welch’s ANOVA with Dunnett’s T3 multiple comparisons test. p < 0.05 was considered statistically significant.

Back to article page