Fig. 3: PCL degradation and thermostability of novel enzymes.
From: Modulating biofilm can potentiate activity of novel plastic-degrading enzymes
a PCL clear zone assay of novel polyester-degrading enzymes. Dh3 and Dh5 are expressed in BL21 (DE3) with zone measurements on day 1, 2 and 3, mean and standard error of the mean (SEM) of 3 biological repeats, empty vector (EV) was used as a control for no PCL clearance. b PCL clear zone images, photographed on day 3, representative image. c Thermostability of secreted Dh3 and Dh5 was assessed by heat treating for 20 minutes and spotting on 1% PCL LB agar plates and incubation at 37 °C. Top row, Dh3 untreated and concentrated, heat-treated at 57.5 °C, 60 °C and 62.5 °C after 24 h. Bottom row, Dh5 untreated and concentrated, heat-treated at 52.5 °C, 55 °C, 57.5 °C, 60 °C after 24 h then 60 °C after 4 days. Representative images of three biological repeats.
