Fig. 5: PCL degradation by polyester-degrading enzymes with increased biofilm formation.
From: Modulating biofilm can potentiate activity of novel plastic-degrading enzymes
a PCL weight loss after 5 days when incubated with BL21 (DE3) empty vector, Dh3 or TfCut2 with and without co-expression of DgcC or WspR. Mean and SEM of seven independent experiments, ordinary one-way ANOVA with multiple comparisons was used to compare whether addition of DGC significantly changed PCL weight loss to the strain without. p-values are 0.0051 for Dh3 vs Dh3 + DgcC and 0.0356 for Dh3 vs Dh3 + WspR, F 7.511. p values 0.0116 for TfCut2 vs TfCut2 + DgcC and 0.0133 for TfCut2 vs TfCut2 + WspR, F 1.429. b Examples of PCL beads from one experiment for Dh3 (top) and TfCut2 (bottom) with and without DgcC and WspR.
