Fig. 6: Representative images of dual-species biofilms (48 h) of Limosilactobacillus reuteri, Enterococcus faecalis, Serratia marcescens and Staphylococcus aureus.
From: Expansion microscopy applied to mono- and dual-species biofilms
Left and middle. Pre-expanded and expanded biofilms as visualized by CLSM. Pre-expanded and expanded biofilms were labelled with SYTO 13 and Alexa Fluor 555 NHS ester, respectively. Scale bars: 10 μm. Inset scale bars: 2 μm. Expansion factors are 3.2 and 4.1 for L. reuteri + E. faecalis; 3.3 for L. reuteri + S. marcescens; 3.4 and 2.1 for L. reuteri + S. aureus; 2.55 and 2.1 for E. faecalis + S. marcescens; 2.8 for E. faecalis + S. aureus and 2.0 and 2.2 for S. marcescens + S. aureus. Right. Non-expanded biofilms as visualized by SEM. Magnifications are ×1000 (×5000, inset) for L. reuteri + E. faecalis, and E. faecalis + S. marcescens; ×500 (×9000, inset) for E. faecalis + S. marcescens and S. marcescens + S. aureus and ×1000 (×9000, inset) for L. reuteri + S. aureus and E. faecalis + S. aureus.
