Fig. 5: hsa-miR-7704 suppressed the growth and adhesion of B. longum via proB inhibition.
a Diversity and b PCA of microbiota in faeces of invitro miR7704-treated and control group. c STAMP was performed on species level. Top17 species screened by fold change and P value were shown. d Relative abundance of faecal microbiota, determined by qPCR. e Correlation between the relative abundance of B. longum/ B. pseudolongum (determined by metagenomics analysis) and the relative expression of hsa-miR-7704 (determined by qPCR) in faeces from patients with HE and CHB (n = 15). Linear regression was performed. f–i Bacteria were grown in the presence of hsa-miR-7704 mimics and scramble (2 µM, 4 h). f Proportions of FAM-hsa-miR-7704 positive bacteria, detected by flow cytometry. g The bacterium (BacLight Red) and miRNA (FAM) were detected and pictured by Imaging streaming. h Growth curve of B. longum. i B. longum pretreated with hsa-miR-7704 mimics or scramble were cultured with HT-29 cells (5 × 107 CFU/mL, 4 h), and gram-stained. Representative images of two different densities are shown. Scale bars, 20 µm. Quantification of the adhering bacteria. j Schematic diagram of the predicted target sites. k Transcripts of the predicted targeting genes, normalized to 16S rRNA. N = 10. l Relative expression of the predicted targeting gene BLLJ_RS09025 [proB] was verified by metagenomics analysis. m Proline levels of miR7704-treated faeces and control. Data were analysed with unpaired Student’s t-test (a, d, i, k, l, m), linear regression analysis (e), or one-way ANOVA (h). Values are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
