Fig. 5: Antibiotic diffusion assay. | npj Biofilms and Microbiomes

Fig. 5: Antibiotic diffusion assay.

From: The skin microbiome stratifies patients with cutaneous T cell lymphoma and determines event-free survival

Fig. 5

The patient derived isolates S. aureus MFMZ1, S. epidermidis MFMZ1, and S. hominis MFMZ1 as well as positive control S. aureus DSM11823 and negative control strains derived from healthy patients S. aureus EM01 and S. epidermidis MV01 were tested on resistance towards a set of commonly used antibiotics in the clinic. a The antibiotics were applied using a Sensi-Disk dispenser with (1) 25 µg ampicillin, (2) 100 µg carbenicillin, (3) (23.75 µg) sulfamethoxazol + (1.25 µg) trimethoprim, (4) 15 µg erythromycin, (5) 10 µg gentamycin, and (6) 5 µg novobiocin. b, c The strains were additionally tested on methicillin resistance using 5 µg and 20 µg methicillin applied on sterile filter disks. The inhibition zone diameter for S. aureus MFMZ1 and DSM11823 strains was ≤ 9 mm, which indicates resistance towards methicillin according to the clinical and laboratory standards institute (CLSI125).

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