Fig. 6: Virulence factors of the MF microbiome. | npj Biofilms and Microbiomes

Fig. 6: Virulence factors of the MF microbiome.

From: The skin microbiome stratifies patients with cutaneous T cell lymphoma and determines event-free survival

Fig. 6

a Differential abundance analysis of the virulence gene repertoire. Displayed are log2 fold-change (log2fc) of virolence genes on patch (n = 19) and plaque (n = 15) compaired to nonlesional (n = 31). b Agarose gel electrophoresis of spa genes from Staphylococci isolates after PCR amplification. From left to right: spa from S. aureus MFMZ1, S. aureus DSM11823 (positive control), S. epidermidis MV01 (negative control), and S. aureus EM01. All detected spa genes are located between the 1500 and 2000 bp line. c ClustalΩ alignment of spa from S. aureus MFMZ1 (spa_MFMZ1) with spa from the S. aureus Newman strain (spa_NM, UniProt ID: A0A0H3K686). spa_MFMZ1 was found to be 24 bp/8 aa longer than spa_NM (1551 bp/516 aa vs. 1527 bp/508 aa) due to the insertion of an additional octapeptide repeat (marked in orange) in the conserved IgG binding domain (marked in green). Furthermore, amino acid variations could be detected in the B-domain (S199A, K215N, marked light blue) and in one of the octapeptide domains of spa_MFMZ1 (N349K, G403N, marked in yellow).

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