Fig. 4: Host specificity and cophylogeny of phages in the bee gut.
From: Host specificity and cophylogeny in the “animal-gut bacteria-phage” tripartite system
a Histogram showing the number of VCs (left y-axis) derived from a single bee species or from multiple bee species at different VC size (x-axis). The VC size refer to the total number of phage and prophage members contained in the VC. The gray line using the right y-axis shows the percentage of VCs derived from a single bee species. b Phylogeny of the three VCs that shows perfect cophylogeny with the bee and bacterial hosts. VC59 and VC413 are from Gilliamella, and VC413 is from Snodgrassella. The neighbor-joining tree is based on dRep distance, with the x-axis representing gANI. c Histogram showing the number of vOTUs (left y-axis) derived from a single bee species or from multiple bee species. d The genetic distance (by dRep software) for phages within the same bee species and for phages between different bee species. The box-and-whisker plots within the violin plots indicate the median and the quartiles. *p < 0.05 by two-sided Student’s t-test. e The relationship between the genetic distance and the variation of gene content for the gut phages. The solid line and the gray area represent the line fit and the 95% confidence interval.
