Table 2 Kinetic parameters of CapGH3b and their respective orthologs

From: A functionally augmented carbohydrate utilization locus from herbivore gut microbiota fueled by dietary β-glucans

Substrate

CapGH3b (this work)

BoGH3b12

BoGH3MLG15

kcat (s−1)

Km*

kcat/Km#

kcat (s−1)

Km*

kcat/Km#

kcat (s−1)

Km*

kcat/Km#

Laminarin (Eisenia bicyclis)a

N.d.

N.d.

-

N.t.

N.t.

-

N.t.

N.t.

-

Laminarin (Laminaria digitata)b

212.3 ± 3.9

1.4 ± 0.1

151.6

N.t.

N.t.

-

N.t.

N.t.

-

β-glucan (from Oat)c

N.d.

N.d.

-

N.t.

N.t.

-

N.t.

N.t.

-

β-glucan (from Yeast)d

N.t.

N.t.

-

N.t.

N.t.

-

N.t.

N.t.

-

pNp-β-Glucopyranoside

60.2 ± 2.1

1.0 ± 0.1

60.2

1.7 ± 0.2

0.16 ± 0.1

11.2

59.5 ± 1.5

3.0 ± 0.1

20.2

Cellobiose (G4G)

13.8 ± 0.1

2.2 ± 0.1

6.3

1.6 ± 0.1

0.7 ± 0.2

2.3

5.5 ± 0.2

7.5 ± 0.5

0.74

Cellotetraose (G4G4G4G)

23.7 ± 1.2

0.6 ± 0.1

37.0

4.0 ± 0.3

0.2 ± 0.1

17.3

17.3 ± 0.5

0.7 ± 0.1

25.2

Cellohexaose (G4G4G4G4G4G)

28.9 ± 1.6

0.5 ± 0.1

61.6

4.9 ± 0.7

0.5 ± 0.2

10.4

17.4 ± 0.4

0.8 ± 0.1

23.3

Laminaribiose (G3G)

203 ± 25

0.6 ± 0.1

370.0

N.t.

N.t.

-

28.0 ± 1.1

1.9 ± 0.1

14.7

Laminaritetraose (G3G3G3G)

232.6 ± 28.6

0.4 ± 0.1

628.7

N.t.

N.t.

-

31.3 ± 2.3

0.9 ± 0.1

34.9

Laminarihexaose (G3G3G3G3G3G)

225.6 ± 34.9

0.5 ± 0.2

501.3

N.t.

N.t.

-

N.t.

N.t.

-

Gentiobiose (G6G)

N.d.

N.d.

-

N.t.

N.t.

-

N.d.

N.d.

0.06

  1. *Km is represented in mM for pNp-β-Glucopyranoside and oligosaccharides and in mg mL−1 for polysaccharides.
  2. #kcat/Km is represented in s−1 mM −1 for pNp-β-Glucopyranoside and oligosaccharides and in s−1 mg−1 mL for polysaccharides.
  3. N.t. not tested, N.d. not detected.
  4. Linkage type: a–β(1,3)-glucan with low β(1–6) substitution [7:1 molar ratio]; b–β(1,3)-glucan with high β(1–6) substitution [3:2 molar ratio]; c–β(1–3)/(1,4)-glucan; and d–β(1,3)-glucan containing long β(1,3)-glucan branches extended from β(1,6)-linked branch points.
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